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standard 96-well microtiter plate

by ryneches, published

standard 96-well microtiter plate by ryneches Sep 16, 2011
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Summary

A standard 96-well microtiter plate with 360 microliter wells.

Dimensions are derived from a Corning 96 Well Microplate.

The PDF describing the mechanical dimensions can be found here :

http://bit.ly/nIWkFX

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13 X 9 = 117
That is one messed up mictotiter plate!

Well thank you for making me google this. That's cool. I hope it'll print waterproof enough for you.

Maybe it is not intended to be printed out, but to serve as a reference for robotics purposes (as in pipetting robots, not the toys)?

Printing microtitre plates has little scientific value, as it is very hard to print with consistent results, so your experiments will have similar inconsistent results. But I could be wrong about that.

Actually, most 3D printers are god enough to produce waterproof prints if they're calibrated correctly. If your printer doesn't work so well, there are plenty of spray-on products that will seal it without much fuss.

As for scientific value, on the contrary. As long as you sterilize and clean them properly, the reaction volume is more than consistent enough (in any event, the precise well volume is not particularly important for most applications).

The real value of 3D printing a microtiter plate is that you
can algorithmicly vary the well volume. I'll post more on that later!

Glad to be wrong then. I assume you are not printing in PLA then? My colleague is churning out beakers and vases, but they do not hold up to the diswasher. What are your procedures for cleaning and sterilising?

I was indeed planning to use PLA, but most of the things I'm printing will be single use. I'm still working on a cleaning protocol, but I was thinking :

[1] Place under UV for 20 minutes
[2] Sonicate in molecular water
[3] Flush with ethanol
[4] Dry under vacuum
[5] Keep under UV in a sealed container until use

I'm mainly concerned with DNA contamination, and don't care about moderate amounts of molecular combinations.

Ah, include a step with lye then if you are concerned about DNA (and/or RNAse) contamination.

But what the F is 'molecular' water? What water is not molecular?

As an alternative technique: maybe you should look into vacuum forming? You can make one mould and form a lot of well plates from that for a much lower price and in shorter time. And since the final material starts as a flat sheet, it is much easier to clean/sterilize.
A vacuum former will set y
ou back ~100$ if you make it yourself. Making moulds I would do on a cnc router. Even a desktop model will do for your purposes.

Heh. It is a funny term, but that's what it's called. "Molecular grade water," as in absolutely pure water (i.e., containing only one kind of molecule). It is to distinguish it from DNA-free water, which may have salts and other inorganic contaminants, di-water, which may have DNA contamination but has had the salts removed, and sterile water, which may have salts and DNA contamination, but in which everything has been thoroughly killed.

Yes, vacuum forming would be a lot cheaper, and I already have a vacuum chamber and house vacuum. The reason I'm using a 3D printer is that I want to programatically set the volume for each individual well for every batch of reactions. I have a script that reads DNA concentrations from a table pro
duced by our plate reader, and generates a custom shape file for the titer plate to dilute them all to the same concentration. This is my way of handling thousands of reactions while avoiding a liquid handling robot.

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