16-well gel electrophoresis combs

by bochman, published

16-well gel electrophoresis combs by bochman Nov 26, 2012
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4830Views 1477Downloads Found in Biology


These combs fit the Bio-Rad vertical mini protein gels using the 0.75, 1.0, or 1.5 mm spacers. Bio-Rad doesn't sell 16-well combs, so they're useful when you have 15 samples and still want to load a molecular weight marker.


I printed these on a piece of blue painter's tape (to facilitate easy removal from the build platform) using a MakerBot Replicator 2. Using MakerWare software, I positioned the combs so that their wide flat side is down and printed using in PLA the default settings: medium quality, 10% infill, 0.2 mm layer height, 2 shells, and 230 degrees C.

UPDATE: The BioRad-advertised thicknesses of these combs is wrong. FOr instance, the 0.75-mm comb is actually only 0.6 mm thick, and that makes a big difference in performance. I've replaced the 0.75-mm file with a 0.6-mm file and the Sketchup file for modifications.

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Hi there!

Thank you for making this file. I would assume you have used the ones you have made, but I just wanted to check because my fear is that the filament used to make the the comb would melt while running the gel, and potentially harm the gel box.




Unless you run your gels differently than me, the comb isn't in the gel while it's running. I was also under the impression that thermoplastics like PLA and ABS melted at a higher temperature than buffer would boil, so if you got your gel rig that hot, you'd have worse things to worry about.