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Histone

by destroyer2012, published

Histone by destroyer2012 Dec 31, 2012

Description

How do you manage a three meter-long molecule if you are a 30 micron-wide cell? Lots of scaffolding proteins. This is a model of a histone complex, which is a complex of eight proteins that work together to organize DNA into a super-coiled structure, sort of like a fractal telephone cord.

From PDB structure 1aoi

Learn more here http://tinyurl.com/azpcjxf

Recent Comments

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If you wonder how to print this darn thing, I just successfully printed it using this same model but sliced in half. Here is the download files..
http://www.thingiverse.com/thing:69152
Thanks! You must get lots of practice printing those actin monomers.
Here is a short video I made about support material. youtube.com/watch?v=bk7KRtJ0WNc

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Instructions

I printed this in PLA, 0.1mm layer height at 50mm/s.

Print with support. I used KISSlicer's dense support function, with the support flow rate reduced to 70% by an external script. The support was still pretty hard, so you may be able to go even lower in the flow rate.

If you wonder how to print this darn thing, I just successfully printed it using this same model but sliced in half. Here is the download files..
http://www.thingiverse.com/thing:69152
histone - supportless
Painting the DNA really makes the structure stand out.
I printed this out yesterday and am still working on the support! (Slic3r, 2.5mm support, default flow rate.) It's definitely worth reducing the flow rate and increasing the spacing as much as you can. I'll post a picture once it looks presentable.
I printed this out yesterday and am still working on the support! (Slic3r, 2.5mm support, default flow rate.) It's definitely worth reducing the flow rate and increasing the spacing as much as you can. I'll post a picture once it looks presentable.
reducing the flow rate is pretty critical in my experience. I have had several PLA prints where the support is fused to the object. This one came pretty close actually.

With ABS the problem is less severe, mostly because the plastic is less sticky and also printed colder relative to its Tg. Getting good supports is striking a balance between support flow rate, fan speed, and extrusion temperature so your object has enough layer adhesion to not delaminate when you remove the supports, but the supports are weak enough to be removed easily.

Another hint is to actually decrease the flow rate only on interface supports (the increased density regions right before the object begins).
This was my first print with support, so I wasn't sure what to expect. Should the support pop off in one piece, or do you usually have to break it up with a hobby knife?

I ended up basically carving a histone out of a semi-solid block of plastic. But the result looks good! I plan on printing more proteins, so I should get lots of practice with support settings.
Here is a short video I made about support material. youtube.com/watch?v=bk7KRtJ0WNc
Thanks! You must get lots of practice printing those actin monomers.
Looks like what my machine produces when the model breaks free of the build platform during a 3 hour print... I never knew it had a name... :)
You should've seen it before I removed the support material
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