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I test a lot of microarray slides and agarose coatings always work well (same stuff you use for DNA electrophoresis gels). So like when you cast DNA gels thought it would be cool to print a really small gel caster for surface coating.
Surprisingly you can make really consistent coatings with this trick and you can use the same set up to attach gaskets to create small wells.
This design is for a 16 pad (8 row x 2 column) set up.
Agarose is cool because you can mix it with different chemistries.
When you finish assay just rehydrate and scrap agarose off
But I haven't been able to get such good data using chemiluminescence, but fluorescence works great.
If you look at the pre and post washes of protein array you can see how well the agarose retains the spots.
Here's more data:
After printing you want make surface waterproof, we glue a small transparency. Then seal the sides with silicon glue.
Dr.RobotLabs DIY (recycleable) microwell plate / microarray slide by htsrjdrouse is licensed under the Creative Commons - Attribution - Share Alike license.
So what's this mean?
We're sure htsrjdrouse would love to see what you've printed - take a photo and share it on Thingiverse as a Make.
To post a Make simply visit this Thing again and click I Made One to start uploading your photo. You can also download the Thingiverse Mobile app (available via Google Play and Apple App Store) to take a photo and upload your Make right from the app!