Plastocyanin (pdb 2CJ3)
by MoleculeMaker, published
This printed well, and is now a completed model. I have painted it and provided some pictures.
This protein is found in the cyanobacterium Anabaena variabilis, and is an important component of the electron transport chain. It has a single copper heteroatom, which I have represented as CPK, along with the two histidine and single cysteine ligands.I tried to print this initially as a smaller protein without supports, but it was too flimsy and broke, so I put in some supports and made a larger model, which printed very well. It is quite sturdy. I painted the atoms in standard colors, with the copper painted magenta and the alpha helices in orange and the beta sheets in teal.
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I printed at Medium Quality using the default settings (10% infill, 2 shells, 0.27 layer height). I included supports, but not a raft (which I later regretted, as it was hard to remove from the base). I started this just after my lunch one overnoon, and then came back at about 9:00 PM, and it was done. I could be remembering this wrong. It is quite a long print, and quite intensive on the PLA that it will use.
Only six hours!? Please, share your feed rate and layer heights.. By my estimate this should take 12 hours at least
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The model took a good deal of effort to construct. The original pdb file (2CJ3) was manipulated until only the single monomer with no water molecules was included. This was then placed into the program VMD. I manipulated the backbone as cartoons, and then selected the histidine and cysteine ligands along with the copper heteroatom for display as CPK, and added some bonds to serve as supports to hold the structure together (these are somewhat flimsy). This was then imported in several segments into tinkercad (tinkercad had a problem importing files larger than 20 Mb, so I had to import and then align as three components. I then grouped and aligned everything, and added a number of manual support features at the bottom. The bottom braces are designed to be removed, while the internal braces hold the model together. I did break a small brace, but glued it back together with some model glue.
If you do choose to print this out, be prepared for a significant level of work, and be patient removing the supports, but the print is very nice. I painted the alpha helices in orange and the beta sheets in teal, and also painted the individual atoms of the copper and ligands.
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