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Unfortunately, it did not work. I receive avrdude timeouts.

avrdude: stk500_2_ReceiveMessage(): timeout

This was from my attempt on windows, which I expect would be the same on the other OS's but can try if it makes sense to do so. Did I get this syntax correct:

tools\avrdude -p m2560 -P COM5: -c stk500v2 -b 57600 -U flash:r:Creator_flash_backup.hex:i

I ran the version of avrdude that came with the replicatorg 40r34 when trying this.