Clamp Loader Complex
by destroyer2012, published
Liked ByView All
Give a Shout Out
If you print this Thing and display it in public proudly give attribution by printing and displaying this tag.Print Thing Tag
The fact is that DNA polymerases are not very processive. They tend to fall off of DNA rather quickly, and because of that they are pretty slow. How to increase the speed and fidelity of a polymerase? You shackle it to the DNA molecule it's replicating, using this.
This is a complex which attaches a sliding clamp around a DNA molecule. Polymerase then sticks to the end of the sliding clamp, and as a result gets stuck fast to the DNA it's replicating, increasing its processivity from 10 base pairs to 80,000.
This is a closed clamp, pdb ID 3u5z.
Print with support. I used 0.4mm layer width and 0.128 layer height (with a 0.4mm nozzle) 45mm/s feed rate. I used KISSlicer and support set to "dense", with the support flow rate reduced to 70% of the object using an external script.
Support was fairly difficult to remove, I used a piece of armature wire to get it out of the cracks and crevices.
Upgrade this Thing with Thingiverse AppsCustomization
Edit, personalize, or revise this ThingPrint Fulfilment
Order a print of this ThingTools and Utilities
Repair, slice, or enhance this Thing
Clamp Loader Complex by destroyer2012 is licensed under the Creative Commons - Attribution - Share Alike license.
What does this mean?
- You must attribute (give credit) to the creator of this Thing.
- You must distribute Remixes under the same license as the original.
- Remixing or Changing this Thing is allowed.
- Commercial use is allowed.
Show Some Love
Say thanks by giving destroyer2012 a tip and help them continue to share amazing Things with the Thingiverse community.Tip Designer
We're sure destroyer2012 would love to see what you've printed. Please document your print and share a Make with the community.
To post a Make simply visit this Thing again and click I Made One to start uploading your photo. It’s even easier to post a Make via the Thingiverse Mobile app (available via Google Play and Apple App Store).